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Related Experiment Videos

Upper intestinal bacterial flora during transpyloric feeding.

H D Dellagrammaticas, B I Duerden, R D Milner

    Archives of Disease in Childhood
    |February 1, 1983
    PubMed
    Summary

    Bacterial colonization in neonates increases with age, with Gram-negative bacteria dominating. Heavy jejunal colonization by Gram-negative bacilli preceded necrotizing enterocolitis in these infants.

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    Area of Science:

    • Microbiology
    • Neonatalogy
    • Gastroenterology

    Background:

    • Neonatal gut microbiome development is crucial for health.
    • Premature infants (<1.5 kg) fed via transpyloric tube tubes have unique microbial exposures.
    • Understanding early microbial colonization patterns is vital for preventing infant diseases.

    Purpose of the Study:

    • To characterize the developing gut microbiota in low-birth-weight neonates.
    • To investigate the relationship between microbial colonization and necrotizing enterocolitis (NEC).

    Main Methods:

    • Collected samples from pharynx, stomach, duodenum/jejunum, and faeces over 28 days.
    • Utilized selective and non-selective media for bacterial culturing.
    • Employed semi-quantitative methods to assess bacterial growth and species diversity.

    Main Results:

    • Bacterial species count and growth density increased with infant age.
    • Gram-negative bacteria showed a notable increase, altering the Gram-negative to Gram-positive ratio.
    • The upper small intestine was colonized earlier and more heavily than the stomach, with a fecal-like microflora.
    • Aerobes and facultative anaerobes predominated; strict anaerobes were not significant.
    • Necrotizing enterocolitis occurred following substantial jejunal Gram-negative bacilli colonization.

    Conclusions:

    • Gut microbial colonization in neonates is dynamic and age-dependent.
    • The shift towards Gram-negative bacteria in the neonatal gut may be a risk factor for NEC.
    • Early and heavy Gram-negative colonization, particularly in the jejunum, is linked to necrotizing enterocolitis development.

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