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Updated: Oct 2, 2026

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques
Published on: July 3, 2014
Replication of five bovine respiratory viruses in cultured bovine alveolar macrophages
Abstract:
The replication of five bovine respiratory viruses in cultured bovine alveolar macrophages (BAM) was investigated. The bovine herpesvirus DN-599 strain did not cause cytophatic effect (CPE), extracellular virus and intracellular antigen were not demonstrated. Although a small number of bovine respiratory syncytial virions were consistently released and about 1 percent of the BAM were fluorescence antibody (FA) positive the virus caused no CPE. Infectious bovine rhinotracheitis virus caused CPE at an input multiplicity of about 0.5, and 5 percent of the BAM were FA positive. Both bovine parainfluenza-3 and bovine viral diarrhea viruses caused CPE, infective virions were released and considerable proportions of the BAM were FA positive.
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