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Related Concept Videos

Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...

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Related Experiment Video

Updated: Jul 10, 2026

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
10:22

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

An experimental model for measuring intestinal permeability.

R Ecknauer, B Buck, D Breitig

    Digestion
    |January 1, 1983
    PubMed
    Summary

    This study measured jejunal permeability in rats using four molecules. Permeability decreased with molecular weight, but was altered by EDTA and deoxycholate, impacting drug delivery research.

    Area of Science:

    • Gastroenterology
    • Pharmacokinetics
    • Physiology

    Background:

    • Jejunal permeability is crucial for nutrient absorption and drug delivery.
    • Understanding factors influencing jejunal permeability is essential for therapeutic development.

    Purpose of the Study:

    • To quantify jejunal permeability to different molecules in non-anesthetized rats.
    • To investigate the effects of specific agents (EDTA, deoxycholate, hydroxyurea) on jejunal permeability.

    Main Methods:

    • Simultaneous administration of mannitol, phenol red, inulin, and polyvinylpyrrolidone (PVP) in rats.
    • Analysis of blood, serum, urine, and duodenal fluid for compound recovery.
    • Assessment of jejunal permeability changes following administration of EDTA, deoxycholate, and hydroxyurea.

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    Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
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    Published on: October 19, 2018

    Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
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    09:48

    Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

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    Main Results:

    • Jejunal permeation rate decreased with increasing molecular weight of the tested substances.
    • Approximately 50% of absorbed molecules were excreted in urine, 50% in extracellular space, and 1% in duodenal juice.
    • EDTA and deoxycholate enhanced permeability for most molecules, while hydroxyurea initially had no effect but later decreased permeability for larger molecules (inulin, PVP).

    Conclusions:

    • Jejunal permeability is inversely related to molecular size.
    • EDTA and deoxycholate can modulate jejunal permeability, suggesting potential therapeutic applications.
    • Hydroxyurea affects jejunal permeability to larger molecules over time, indicating an impact on mucosal integrity.