Combined effect of lysine and acetate on gluconeogenesis from lactate in isolated hepatocytes

The effect of lysine combined with acetate on gluconeogenesis from lactate was studied in isolated rat hepatocytes. The combination was found to have a powerful stimulative effect which was significantly greater than that of isolated lysine or acetate. The stimulative effects of the two stimulants were mutually additive and were correlated [both singly and combined] to the free NADH and NAD+ concentration ratio in the cytoplasm.