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The micronucleus test with mouse spleen cells.

Y Shindo, F Hirano, H Maeda

    Mutation Research
    |July 1, 1983
    PubMed
    Summary
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    The mouse micronucleus test is effective using spleen cells, comparable to bone marrow. Certain compounds induced genetic damage in both tissues, while the antibiotic fosfomycin did not.

    Area of Science:

    • Toxicology
    • Genetics
    • Hematology

    Background:

    • The micronucleus test is a standard method for assessing genotoxicity.
    • Traditionally, bone marrow cells are used, but alternative tissues are being explored.

    Purpose of the Study:

    • To evaluate the efficacy of the mouse spleen as an alternative to bone marrow for the micronucleus test.
    • To compare the frequency of micronucleated polychromatic erythrocytes in spleen and bone marrow cells.

    Main Methods:

    • Mice were administered three test compounds: Mitomycin C, cyclophosphamide, and fosfomycin.
    • Micronucleated polychromatic erythrocytes were analyzed in both spleen and bone marrow samples.
    • Frequencies of polychromatic erythrocytes were determined in both tissues.

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    Main Results:

    • Polychromatic erythrocytes constituted approximately 9% of spleen cells and 13% of bone marrow cells.
    • Mitomycin C and cyclophosphamide induced micronucleated polychromatic erythrocytes in both spleen and bone marrow.
    • Fosfomycin did not induce micronucleated erythrocytes in either the spleen or bone marrow.

    Conclusions:

    • The mouse spleen is a viable alternative tissue for conducting the micronucleus test.
    • The spleen assay demonstrated sensitivity to genotoxic agents like Mitomycin C and cyclophosphamide.
    • Fosfomycin exhibited no genotoxic potential in this in vivo micronucleus test system.