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[Cell biology basis of IgE antibody regulation. Immunotherapeutic approaches]
Recent developments as to the IgE-antibody synthesis in rodents and in the human model are summarized and discussed in reference to our own data. It is by now established that the IgE-antibody response is regulated by interdependent interactions of cellular, humoral and genetic factors. The IgE-bearing B-lymphocyte develops from a surface IgM carrying B-lymphocyte. The transformation into an IgE secreting plasma cell requires T-cell help or soluble T-cell factors. Recent advances in the characterization of human lymphocytes as well as more sophisticated cell biological approaches facilitated the analysis of IgE-antibody synthesis in the human in vitro model. In most of the studies at present available the effect of the polyclonal B-cell mitogen (PWM) was analyzed. PWM either enhanced, suppressed or unlike to other Ig-classes did not affect the IgE-antibody synthesis at all. Immunotherapeutic approaches have to consider to establish the allergen induced in vitro model of IgE-antibody synthesis in humans, to modulate the interactions of T-helper and/or suppressor cells or to generate soluble suppressor factors. In any case the analysis of the IgE-antibody synthesis in humans could be a valuable tool to assess IgE-B-cell memory towards various allergens and to determine the IgE-mediated in patients.
Recent developments as to the IgE-antibody synthesis in rodents and in the human model are summarized and discussed in reference to our own data. It is by now established that the IgE-antibody response is regulated by interdependent interactions of cellular, humoral and genetic factors. The IgE-bearing B-lymphocyte develops from a surface IgM carrying B-lymphocyte. The transformation into an IgE secreting plasma cell requires T-cell help or soluble T-cell factors. Recent advances in the characterization of human lymphocytes as well as more sophisticated cell biological approaches facilitated the analysis of IgE-antibody synthesis in the human in vitro model. In most of the studies at present available the effect of the polyclonal B-cell mitogen (PWM) was analyzed. PWM either enhanced, suppressed or unlike to other Ig-classes did not affect the IgE-antibody synthesis at all. Immunotherapeutic approaches have to consider to establish the allergen induced in vitro model of IgE-antibody synthesis in humans, to modulate the interactions of T-helper and/or suppressor cells or to generate soluble suppressor factors. In any case the analysis of the IgE-antibody synthesis in humans could be a valuable tool to assess IgE-B-cell memory towards various allergens and to determine the IgE-mediated in patients.