Lipoxygenase from rabbit reticulocytes: iron content, amino acid composition and C-terminal heterogeneity

Purified lipoxygenase from rabbit reticulocytes contains 1 g atom iron per molecule. The amino acid composition shows that the enzyme contains 11 cysteine residues but no disulfide bridges and contains a relatively high content of hydrophobic amino acids. The enzyme shows heterogeneity in the C-terminus. With the tritium labeling method the amino acids His, Asn and Ile were found in the C-terminal region. The absorption co-efficient was found to be 1.68 (E0.1% 280 1 cm) in the native state and 1.77 in the unfolded state.