Aggregation of platelets from monocrotaline pyrrole-treated rats

Aggregation responses of platelet-rich plasma (PRP) from monocrotaline pyrrole (MCTP)-treated rats were examined to help elucidate the role of platelets in MCTP-induced pulmonary hypertension. PRP from rats treated one or four days earlier with MCTP (5 mg/kg, i.v.) or vehicle exhibited the same slope and maximum aggregation to ADP (1 X 10(-5) M, 2 X 10(-6) M, 1 X 10(-6) M), 74 micrograms/ml dog collagen, or 500 micrograms/ml arachidonic acid. PRP collected 7 days after MCTP treatment had a 15% decrease in both slope and maximum aggregation to 1 X 10(-5) M ADP and a 25% decrease in response to 2 X 10(-6) M ADP relative to the control group. Fourteen days after MCTP treatment, the responses to all of the aggregating agents were decreased 18-71% from control values. These results indicate that MCTP treatment alters the responses of PRP to aggregating agents.