Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein binding of enprofylline.

K Tegnér, O Borgå, I Svensson

    European Journal of Clinical Pharmacology
    |January 1, 1983
    PubMed
    Summary

    Enprofylline, a 3-propylxanthine derivative, exhibits moderate plasma protein binding (47.3%) in humans, similar to theophylline. Binding significantly increases with pH and shows notable species-dependent variations, particularly between rodents and primates.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Forces applied at the footrest during ergometer kayaking among female athletes at different competing levels - a pilot study.

    BMC sports science, medicine & rehabilitation·2019
    Same author

    Oxidant-induced apoptosis: a consequence of lethal lysosomal leak?

    Redox report : communications in free radical research·2016
    Same author

    A dose-finding study with a novel water-soluble formulation of paclitaxel for the treatment of malignant high-grade solid tumours in dogs.

    Veterinary and comparative oncology·2012
    Same author

    Grapefruit juice interaction with oral budesonide: equal effect on immediate-release and delayed-release formulations.

    Die Pharmazie·2009
    Same author

    Effect of erythromycin on the absorption of fexofenadine in the jejunum, ileum and colon determined using local intubation in healthy volunteers.

    International journal of clinical pharmacology and therapeutics·2006
    Same author

    Effects of HI 6, diazepam and atropine on soman-induced IL-1 beta protein in rat brain.

    Neurotoxicology·2005

    Area of Science:

    • Pharmacology
    • Biochemistry
    • Drug Metabolism and Pharmacokinetics

    Background:

    • Understanding drug-protein binding is crucial for predicting pharmacokinetic behavior and therapeutic efficacy.
    • Enprofylline, a xanthine derivative, requires characterization of its plasma protein binding properties.
    • Xanthine derivatives like theophylline exhibit pH-dependent protein binding.

    Purpose of the Study:

    • To investigate the extent and characteristics of enprofylline protein binding in plasma.
    • To compare enprofylline binding with that of theophylline.
    • To assess the influence of pH and species on enprofylline plasma protein binding.

    Main Methods:

    • Equilibrium dialysis and ultrafiltration were employed to determine protein binding.
    • Experiments were conducted under varied conditions, including different pH levels.
    • Plasma samples from multiple species (human, monkey, dog, rat) were utilized.

    Main Results:

    • Mean enprofylline binding in human plasma at 20°C was 47.3%, slightly lower than theophylline.
    • Both drugs showed a dramatic increase in binding between pH 7.2 and 7.8.
    • Significant species differences were observed: 57% (dog), 81% (rat), 47% (human), and 48% (monkey) at pH 7.4.

    Conclusions:

    • Enprofylline exhibits moderate and pH-dependent plasma protein binding in humans.
    • Species variation in enprofylline binding is pronounced, with higher binding in rodents compared to primates.
    • Equilibrium dialysis is a reliable method, while ultrafiltration presented practical challenges for pH control.

    Related Experiment Videos