Related Experiment Video

Updated: Sep 14, 2026

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
08:46

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Published on: July 12, 2017

The flow cytometry for diagnosis of male fertility

W Hartmann, H Hettwer

    Acta Histochemica
    |January 1, 1983
    PubMed

    Abstract:

    The patient came with a severe testicular disturbance (oligoasthenoteratozoospermia) into the department of andrology. After antiphlogistic- and antiestrogen therapy the clinical spermatological findings (spermiograms) were compared with the corresponding histograms of the flow cytometry analysis and interpreted as according with them.

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    A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
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    Published on: July 12, 2017

    Fluorimetric Techniques for the Assessment of Sperm Membranes
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    Published on: November 28, 2018

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    Flow Cytometry01:23

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    The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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    Infertility in Males01:23

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    Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...

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