Omega-hydroxylation of 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid in human polymorphonuclear leukocytes

Incubation of arachidonic acid or 12-Ls-hydroxy-5,8,10,14-eicosatetraenoic acid with suspensions of human polymorphonuclear leukocytes led to the formation of 12-Ls,20-dihydroxy-5,8,10,14-eicosatetraenoic acid. The structure of the new metabolite was by physical methods and by chemical degradation.