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Related Experiment Videos

Rabbit small intestinal beta-galactosidases.

F Toofanian

    American Journal of Veterinary Research
    |March 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Rabbit small intestinal mucosa contains four beta-galactosidases, including a neutral form (lactase) and three acid forms. Three enzymes align with those in other mammals, but one hetero-beta-galactosidase is novel.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Mammalian Physiology

    Background:

    • Beta-galactosidases are crucial enzymes involved in carbohydrate metabolism.
    • Understanding the diversity of beta-galactosidases in different species aids in comprehending digestive processes and evolutionary adaptations.

    Purpose of the Study:

    • To characterize the beta-galactosidase activities present in rabbit small intestinal mucosa.
    • To determine the optimal pH and substrate specificities of identified beta-galactosidases.
    • To investigate the presence of novel beta-galactosidase forms in rabbits.

    Main Methods:

    • Enzyme assays were performed on rabbit small intestinal mucosa extracts.
    • Activity was measured across a pH range of 2.6 to 8.4 using various substrates.

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  • The effect of the enzyme inhibitor p-chloromercuribenzoic acid was assessed.

    • Main Results:

    • Four distinct beta-galactosidase activities were identified.
    • These included a neutral beta-galactosidase (lactase) with an optimal pH of 5.8.
    • Three acid beta-galactosidases (I and II) with optimal pHs of 3.4 and 4.6, and a hetero-beta-galactosidase with an optimal pH of 7.6 were also found.

    Conclusions:

    • Rabbit small intestine possesses at least four types of beta-galactosidases.
    • Three of these enzymes show similarities to those found in other mammalian species.
    • A novel hetero-beta-galactosidase, not previously reported in other species, was identified in rabbits.