Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The adaptable somatic repertoire.

P J Gearhart

    Annales D'Immunologie
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Somatic hypermutation introduces nucleotide substitutions in the Vk167 gene, likely via error-prone repair during cell division. Variants of anti-phosphorylcholine antibodies often show enhanced antigen affinity compared to original versions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Generation of immunoglobulin variable gene diversity.

    Immunology today·2014
    Same author

    Emerging links between hypermutation of antibody genes and DNA polymerases.

    Nature reviews. Immunology·2002
    Same author

    Third complementarity-determining region of mutated VH immunoglobulin genes contains shorter V, D, J, P, and N components than non-mutated genes.

    Immunology·2001
    Same author

    Analysis of microsatellite instability and hypermutation of immunoglobulin variable genes in Werner syndrome.

    Mechanisms of ageing and development·2001
    Same author

    Altered nucleotide misinsertion fidelity associated with poliota-dependent replication at the end of a DNA template.

    The EMBO journal·2001
    Same author

    DNA polymerase eta is an A-T mutator in somatic hypermutation of immunoglobulin variable genes.

    Nature immunology·2001
    Same journal

    Lymphocytes migration into membrane filters: divergent effects of syngeneic mouse serum and lymph.

    Annales d'immunologie·1984
    Same journal

    Maternal antibody to foetal histocompatibility and trophoblast-specific antigens.

    Annales d'immunologie·1984
    Same journal

    Invasive trophoblast in the genus Equus.

    Annales d'immunologie·1984
    Same journal

    Effect of mycobacterial lipids on membrane fluidity and natural killer cell-mediated cytotoxicity.

    Annales d'immunologie·1984
    Same journal

    Heterogeneity of human B lymphocytes as revealed by monoclonal antibodies.

    Annales d'immunologie·1984
    Same journal

    Effects of T mitogens on in vivo antibody production to a T-dependent antigen in lines of mice genetically selected for high or low in vitro responsiveness to PHA.

    Annales d'immunologie·1984
    See all related articles

    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • Somatic hypermutation is crucial for antibody diversity.
    • Understanding the mechanisms driving antibody gene mutation is essential.

    Purpose of the Study:

    • To investigate somatic mutation patterns in the rearranged kappa variable gene, Vk167.
    • To explore the relationship between mutation accumulation and antibody class (IgM, IgG, IgA).
    • To assess the impact of mutations on antibody affinity for antigen.

    Main Methods:

    • Analysis of nucleotide sequences in Vk167 and its constant gene.
    • Comparison of mutation frequencies across different antibody isotypes.
    • Affinity measurements for variant anti-phosphorylcholine antibodies.

    Related Experiment Videos

    Main Results:

    • Single nucleotide substitutions were observed in and around the Vk167 gene, but not the constant gene.
    • High mutation frequency suggests an error-prone repair mechanism during cell division.
    • Accumulation of mutations correlates with time after V-J joining, with IgG and IgA showing more substitutions than IgM.
    • Many variant anti-phosphorylcholine antibodies exhibited higher antigen affinity than germline counterparts.

    Conclusions:

    • Error-prone repair is a likely driver of somatic mutations in Vk167.
    • Mutation accumulation over time influences antibody isotype and affinity.
    • Antibody variants can achieve superior antigen binding through somatic hypermutation.