Related Experiment Videos
Antithrombin III determination by rate (kinetic) nephelometry.
Thrombosis and Haemostasis
|February 28, 1984
Summary
A new rate nephelometry method offers rapid immunological determination of antithrombin III. This method shows strong correlation with established techniques, making it a viable diagnostic tool.
Area of Science:
- Clinical chemistry
- Immunology
- Biochemical analysis
Background:
- Antithrombin III (ATIII) is a crucial protein in the coagulation cascade.
- Accurate and rapid measurement of ATIII levels is essential for diagnosing and managing thrombotic disorders.
Purpose of the Study:
- To develop and validate a new rate nephelometry method for immunological antithrombin III determination.
- To assess the efficiency and correlation of the new method with existing techniques.
Main Methods:
- Utilized the Beckman Immunochemistry System for rate nephelometry.
- Employed a small plasma volume (20 microliters) for each measurement.
- Compared results with radial immunodiffusion and Coatest (Kabi-Ortho) methods.
Main Results:
- The assay provides results within 60 seconds.
- Demonstrated a high correlation with radial immunodiffusion (r = 0.91).
- Showed a good correlation with Coatest (r = 0.77).
Conclusions:
- The developed rate nephelometry method is a rapid and reliable technique for antithrombin III determination.
- This method is suitable for clinical laboratory use, offering quick results.
- Both laser-nephelometric and non-nephelometric anti-ATIII sera are compatible with the system.