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[Isolation and several properties of neurospecific antigen D]
Ukrainskii Biokhimicheskii Zhurnal (1978)
|March 1, 1984
Summary
Researchers isolated neurospecific protein antigen D from bull brain tissue. This protein, identified as the gamma-type isoform of enolase, exhibits enolase activity and is identical to protein 14-3-2.
Area of Science:
- Neurochemistry
- Protein Biochemistry
- Enzymology
Context:
- Neurospecific proteins play crucial roles in brain function.
- Antigen D is a protein found in brain tissue.
- Understanding protein identity is key to elucidating biological functions.
Purpose:
- To isolate and characterize neurospecific protein antigen D from bull brain tissue.
- To determine the enzymatic activity and electrophoretic properties of antigen D.
- To compare antigen D with known brain proteins, specifically protein 14-3-2.
Summary:
- Neurospecific protein antigen D was isolated and purified from bull brain using polyacrylamide gel electrophoresis (PAAG).
- The molecular mass of antigen D was determined to be 53 kDa.
- Purified antigen D demonstrated enolase activity, corresponding to the gamma-type isoform of this enzyme.
Impact:
- The study establishes the identity of antigen D with protein 14-3-2 based on shared electrophoretic, immunologic, and enzymatic characteristics.
- This finding contributes to the understanding of brain-specific proteins and their functions.
- Identifies a specific enolase isoform in the brain, potentially relevant for neurological research.