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In vivo studies of 111In-labelled canine lymphocytes.

L W Robinson, S D Yeh, R S Benua

    International Journal of Nuclear Medicine and Biology
    |January 1, 1984
    PubMed
    Summary
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    Indium-111 labeled lymphocytes effectively visualized canine lymph nodes and tumors. This technique shows promise for assessing immune cell activity in vivo, aiding in cancer detection and staging.

    Area of Science:

    • Nuclear Medicine
    • Immunology
    • Veterinary Oncology

    Background:

    • Lymphocyte tracking is crucial for understanding immune responses and diagnosing diseases.
    • In vivo imaging of radiolabeled cells offers a non-invasive method for monitoring cell distribution.

    Purpose of the Study:

    • To evaluate the utility of Indium-111 oxine labeled canine peripheral blood lymphocytes for in vivo imaging.
    • To assess the ability of this method to visualize regional lymph nodes and tumor sites in dogs.

    Main Methods:

    • Canine peripheral blood lymphocytes were labeled with Indium-111 oxine.
    • Cell viability and labeling efficiency were assessed.
    • Gamma camera imaging was performed 24-44 hours post-injection in normal dogs and a dog with breast carcinoma.

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    Main Results:

    • High cell viability (approx. 95%) and good labeling efficiency (40-75%) were achieved.
    • Images revealed uptake in subcutaneous lymph nodes, liver, spleen, bowel, and bone marrow.
    • In the tumor-bearing dog, uptake was observed in the tumor, lungs, and axillary lymph node.

    Conclusions:

    • Indium-111 labeled lymphocytes are effective for visualizing regional lymph nodes in dogs.
    • This technique may be valuable for in vivo assessment of tumor recognition by lymphocytes.
    • The method shows potential for veterinary diagnostic applications in oncology.