Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzymatic-ultraviolet method for measuring lactose in milk: collaborative study.

D H Kleyn, J R Trout

    Journal - Association of Official Analytical Chemists
    |May 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A randomized, open-label, multicenter, phase II study evaluating the efficacy and safety of BTH1677 (1,3-1,6 beta glucan; Imprime PGG) in combination with cetuximab and chemotherapy in patients with advanced non-small cell lung cancer.

    Investigational new drugs·2017
    Same author

    Iron sucrose causes greater proteinuria than ferric gluconate in non-dialysis chronic kidney disease.

    Kidney international·2007
    Same author

    Ultrase MT12 and Ultrase MT20 in the treatment of exocrine pancreatic insufficiency in cystic fibrosis: safety and efficacy.

    Alimentary pharmacology & therapeutics·2004
    Same author

    Determination of fat in raw and processed milks by the Gerber method: collaborative study.

    Journal of AOAC International·2001
    Same author

    Metaanalysis of the safety and efficacy of an adhesion barrier (Interceed TC7) in laparotomy.

    The Journal of reproductive medicine·1999
    Same author

    The rates of adhesion development and the effects of crystalloid solutions on adhesion development in pelvic surgery.

    Fertility and sterility·1998
    Same journal

    GC/MIP/AED method for pesticide residue determination in fruits and vegetables.

    Journal - Association of Official Analytical Chemists·1991
    Same journal

    Separation and determination of diesel contaminants in various fish products by capillary gas chromatography.

    Journal - Association of Official Analytical Chemists·1991
    Same journal

    Analysis of organochlorine pesticide residues using simultaneous injection of two capillary columns with electron capture and electrolytic conductivity detectors.

    Journal - Association of Official Analytical Chemists·1991
    Same journal

    Improved method for determination of polynuclear aromatic hydrocarbons in pharmacopoeial paraffin and mineral oils.

    Journal - Association of Official Analytical Chemists·1991
    Same journal

    Monitoring aldehyde production during frying by reversed-phase liquid chromatography.

    Journal - Association of Official Analytical Chemists·1991
    Same journal

    Use of the Mycosep multifunctional cleanup column for liquid chromatographic determination of aflatoxins in agricultural products.

    Journal - Association of Official Analytical Chemists·1991
    See all related articles

    An enzymatic method for lactose determination in milk was found to be less accurate than the official AOAC gravimetric method. This dairy analysis method showed significant differences, indicating a lack of agreement for accurate lactose content measurement.

    Area of Science:

    • Food Science
    • Analytical Chemistry
    • Dairy Science

    Background:

    • Accurate lactose determination is crucial for the dairy and food industries.
    • Existing methods require validation for routine use.

    Purpose of the Study:

    • To evaluate the performance of an enzymatic method for lactose determination in milk.
    • To compare the enzymatic method against the official AOAC gravimetric method.

    Main Methods:

    • Eight dairy and food laboratories analyzed unknown milk samples (milk, lowfat, skim) in blind duplicates.
    • Samples were analyzed using an enzymatic method and the official AOAC gravimetric method (16.507).

    Main Results:

    • The enzymatic method averaged 0.49% lower lactose content than the AOAC method (P = 0.05).

    Related Experiment Videos

  • Significant variations in results were observed between laboratories.
  • Standard deviations for blind duplicates were consistent, ranging from 3.67% to 4.55% lactose.

    • Conclusions:

    • The enzymatic method showed a lack of agreement with the AOAC method for lactose determination.
    • Inter-laboratory variations suggest potential challenges in method standardization.
    • The method was adopted official first action despite observed discrepancies.