Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tetrahymena histone H3. Purification and two variant sequences.

T Hayashi, H Hayashi, Y Fusauchi

    Journal of Biochemistry
    |June 1, 1984
    PubMed
    Summary

    The H3 histone from Tetrahymena pyriformis was sequenced, revealing two variants (H3(1) and H3(2)) with distinct amino acid sequences. These findings offer insights into histone evolution and structure-function relationships.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    5-HT3 receptor antagonists. 1. New quinoline derivatives.

    Journal of medicinal chemistry·1992
    Same author

    New bronchodilators. II. 3H-imidazo[4,5-c]quinolin-4(5H)-ones.

    Chemical & pharmaceutical bulletin·1992
    Same author

    Site of attachment of mercuribenzoate in crystals of an actin:DNase I complex.

    Journal of biochemistry·1992
    Same author

    Further studies on aspartate aminotransferase of thermophilic methanogens by analysis of general properties, bound cofactors, and subunit structures.

    Journal of biochemistry·1992
    Same author

    Elevated expression of the ornithine decarboxylase gene in human esophageal cancer.

    Cancer research·1992
    Same author

    Experimental chemotherapy for xenograft cell lines of human bile duct and gall bladder cancers in nude mice.

    Journal of surgical oncology·1992

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Evolutionary Biology

    Background:

    • Histones are crucial for DNA packaging and gene regulation.
    • The H3 histone variant in the protozoan Tetrahymena pyriformis was previously isolated.

    Purpose of the Study:

    • To completely determine the amino acid sequences of the two H3 histone variants (H3(1) and H3(2)) from Tetrahymena pyriformis.
    • To compare these sequences with human H3 histone and analyze their evolutionary implications.

    Main Methods:

    • Purification of H3 histone using Sephadex G-50 chromatography.
    • Direct N-terminal sequencing by Edman degradation.
    • Sequence determination of tryptic peptides and cyanogen bromide fragments.

    Main Results:

    • The H3 histone mixture comprised 75% H3(1) and 25% H3(2).
    • Complete amino acid sequences for both H3(1) and H3(2) (135 residues each) were determined.
    • H3(1) and H3(2) differ in 14 positions and show significant divergence from human H3.

    Conclusions:

    • The determined sequences provide a basis for understanding H3 histone structure-function relationships in protozoa.
    • Sequence comparisons suggest evolutionary divergence of histone proteins across different species.
    • Post-translational modifications (acetylation, methylation) occur similarly in both variants at six lysine residues.

    Related Experiment Videos