[The use of immunological method for the determination of acid alpha-glucosidase activity in human muscles]

Homogeneous preparation of acid alpha-glucosidase, obtained from human placenta, was used for immunization of rabbits and production of specific antiserum. Immunological procedure was developed for estimation of acid alpha-glucosidase activity from human muscles in presence of the enzyme neutral form. Activity of acid alpha-glucosidase was measured in 20 patients with various types of nervous system impairments. Total activity of alpha-glucosidase in muscles of the patients was 11.4 +/- 0.7 nM/min/g of the tissue and activity of the acid form constituted about 84.8 +/- 1.7% of the total activity.