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Supramolecular structures in mycoplasmas.

U Göbel

    The Yale Journal of Biology and Medicine
    |September 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Mycoplasma pneumoniae possesses a detergent-resistant cytoskeleton composed of microfilaments resembling eukaryotic actin. These filaments are crucial for motility, forming distinct structures in motile cells but absent in nonmotile mutants.

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    Area of Science:

    • Microbiology
    • Cell Biology
    • Biochemistry

    Background:

    • Mycoplasma pneumoniae is a bacterium known for its unique cellular structure and motility.
    • The presence and function of cytoskeletal elements in prokaryotes are of significant interest.
    • Eukaryotic actin filaments play critical roles in cell structure, motility, and division.

    Purpose of the Study:

    • To investigate the presence and nature of a cytoskeleton in Mycoplasma pneumoniae.
    • To determine if the cytoskeletal components share similarities with eukaryotic actin.
    • To explore the relationship between cytoskeletal structure and cell motility in Mycoplasma pneumoniae.

    Main Methods:

    • Treatment of Mycoplasma pneumoniae cells with Triton X-100 to isolate detergent-resistant structures.

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  • Morphological and chemical characterization of the cytoskeleton, including staining with anti-actin antibodies and rhodamine-labeled phalloidin.
  • Isolation of microfilaments via differential centrifugation and analysis by SDS gel electrophoresis.
  • Comparison of isolated proteins with known standards, such as rabbit muscle actin.

    • Main Results:

    • A detergent-resistant cytoskeleton was identified in Mycoplasma pneumoniae cells.
    • The cytoskeleton consists of microfilaments exhibiting morphological and chemical similarities to eukaryotic actin, including positive staining with anti-actin antibodies and rhodamine-labeled phalloidin.
    • A protein of 42.5 kDa co-migrated with rabbit muscle actin.
    • These filaments were absent in a nonmotile mutant (M-22), suggesting a role in motility.
    • Filament arrangement differed between the cell body and the leading edge of motile cells.

    Conclusions:

    • Mycoplasma pneumoniae possesses a cytoskeleton containing actin-like microfilaments.
    • These microfilaments are likely involved in the motility of Mycoplasma pneumoniae.
    • While showing similarities, the precise degree of homology between these bacterial microfilaments and eukaryotic actin requires further investigation.