Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in phosphatidylserine synthesis in brain microsomes of Shambling mutant mouse.

T Yamaguchi, Y Matsumura, M Yamaguchi

    Biochemistry International
    |March 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Preparation of a fluorescent derivative of benzoylecgonine, and preliminary studies of its application to the analysis of urine.

    Journal of chromatography·1992
    Same author

    [A case of insulinoma with frequent hypoglycemic attacks not showing evident hyperinsulinemia].

    Nihon Naibunpi Gakkai zasshi·1992
    Same author

    High-performance liquid chromatographic determination of fatty acid binding proteins in rat liver with fluorescence detection.

    The Analyst·1992
    Same author

    [A case of allergic bronchopulmonary aspergillosis with no history of bronchial asthma].

    Nihon Kyobu Shikkan Gakkai zasshi·1992
    Same author

    Disseminated intravascular coagulation in lupus erythematosus with acute liver damage.

    Internal medicine (Tokyo, Japan)·1992
    Same author

    Expansion of a paroxysmal nocturnal hemoglobinuria (PNH) clone after cyclosporine therapy for aplastic anemia/PNH syndrome.

    Blood·1992
    Same journal

    Monitoring of haemoglobin-methyl isocyanate adduct by high-performance liquid chromatography with diode array detector.

    Biochemistry international·1992
    Same journal

    Intervention of adriamycin induced free radical damage.

    Biochemistry international·1992
    Same journal

    Relationship between substrate activity and pKa value of phenols on sulfotransferase from Eubacterium A-44.

    Biochemistry international·1992
    Same journal

    Purification and properties of chitinase from cabbage.

    Biochemistry international·1992
    Same journal

    Coordinate expression of the enzymes of nitric oxide metabolism in Paracoccus denitrificans.

    Biochemistry international·1992
    Same journal

    Characterization of trehalase in Rhodotorula rubra.

    Biochemistry international·1992
    See all related articles

    Shambling mutant mice show reduced phosphatidylserine (PS) synthesis in the brain. This defect is linked to calmodulin-dependent synthesis and can be partially corrected by thyrotropin-releasing hormone (TRH).

    Area of Science:

    • Neurochemistry
    • Biochemistry
    • Genetics

    Background:

    • Phosphatidylserine (PS) is crucial for neuronal function and membrane integrity.
    • The Shambling mutant mouse exhibits ataxia, suggesting neurological dysfunction.
    • Calmodulin plays a role in regulating various cellular processes, including lipid synthesis.

    Purpose of the Study:

    • To investigate the rate of phosphatidylserine (PS) synthesis in the brain microsomes of ataxic and heterozygous Shambling mutant mice.
    • To determine the role of calmodulin in the observed decrease in PS synthesis.
    • To explore the potential of thyrotropin-releasing hormone (TRH) in modulating PS synthesis and compensating for the genetic defect.

    Main Methods:

    • Measurement of phosphatidylserine (PS) synthesis rates in brain microsomes from control and Shambling mutant mice.

    Related Experiment Videos

  • Assay of PS synthesis in the presence of varying calmodulin concentrations.
  • In vitro assessment of TRH's effect on PS synthesis rates.

    • Main Results:

    • Ataxic Shambling mutant mice displayed a 40% reduction in brain PS synthesis compared to controls.
    • Heterozygous nonataxic mutants showed an 80% rate of PS synthesis.
    • The reduction in PS synthesis in ataxic mutants correlated with calmodulin-sensitive PS synthesis in control brains.
    • Thyrotropin-releasing hormone (TRH) significantly enhanced PS synthesis rates in vitro, compensating for the genetic defect.

    Conclusions:

    • The genetic defect in Shambling mutant mice leads to a significant reduction in brain phosphatidylserine (PS) synthesis.
    • Calmodulin-dependent pathways are implicated in the PS synthesis deficit.
    • Thyrotropin-releasing hormone (TRH) shows potential as a therapeutic agent to restore PS synthesis and potentially ameliorate motor deficits.