Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Site-specific carcinogen binding to DNA.

T C Boles, M E Hogan

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Allelopathy of small everlasting (Antennaria microphylla) : Phytotoxicity to leafy spurge (Euphorbia esula) in tissue culture.

    Journal of chemical ecology·2013
    Same author

    Differential allelochemical detoxification mechanism in tissue cultures ofAntennaria microphylla andEuphorbia esula.

    Journal of chemical ecology·2013
    Same author

    Effect of temperature and sucrose concentration on hydroquinone toxicity in leafy spurge suspension culture cells.

    Journal of chemical ecology·2013
    Same author

    Varieties of positive and negative priming.

    Psychonomic bulletin & review·2013
    Same author

    Impairment in the achievement domain in bipolar spectrum disorders: role of behavioral approach system hypersensitivity and impulsivity.

    Minerva pediatrica·2008
    Same author

    Childhood maltreatment and college students' current suicidal ideation: a test of the hopelessness theory.

    Suicide & life-threatening behavior·2002
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Environmental carcinogen benzo[alpha]pyrene diol epoxide (BPDE) preferentially binds to specific DNA regions. A new photochemical method maps these binding sites, revealing nonrandom distribution within the chicken beta-globin gene.

    Area of Science:

    • Environmental Science
    • Molecular Biology
    • Genetics

    Background:

    • Benzo[alpha]pyrene diol epoxide (BPDE) is a known environmental carcinogen that forms covalent DNA adducts.
    • Understanding the precise location of BPDE-DNA binding is crucial for elucidating its carcinogenic mechanisms.

    Purpose of the Study:

    • To develop and apply a novel photochemical technique for mapping BPDE-binding sites in cloned DNA sequences.
    • To investigate the distribution of BPDE adducts within the chicken adult beta-globin gene.

    Main Methods:

    • A photochemical method utilizing 355 nm laser irradiation to induce single-strand DNA cuts at BPDE binding sites.
    • Analysis of DNA from cloned chicken adult beta-globin gene treated with BPDE.

    Main Results:

    Related Experiment Videos

    • The study successfully mapped BPDE-binding sites within the chicken beta-globin gene.
    • BPDE binding was found to be nonrandom, with a 300-base-pair region 5' to the RNA cap site showing the highest susceptibility.
    • This highly targeted region is known to contain critical transcriptional control sequences.

    Conclusions:

    • The developed photochemical technique provides a precise method for mapping carcinogen-DNA adducts.
    • Sequence-specific binding of BPDE to regulatory elements, particularly near the RNA cap site, may be a key factor in its genotoxicity and carcinogenicity.