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Inter- and intra-strain variants in the genus Brucella
Developments in Biological Standardization
|January 1, 1984
Summary
This study investigated bacterial strain variation using conventional and manometric methods. Brucella suis and Brucella abortus showed significant variation, while Brucella melitensis and Brucella neotomae exhibited stability.
Area of Science:
- Microbiology
- Bacteriology
- Genetics
Background:
- Bacterial identification and classification rely on understanding strain variation.
- Brucella species are significant pathogens with diverse biotypes and hosts.
- Assessing intra- and inter-strain variability is crucial for accurate identification and epidemiological studies.
Purpose of the Study:
- To determine inter- and intra-strain variation within Brucella species and biotypes.
- To compare conventional identification methods with manometric analysis of oxidative metabolic patterns.
- To investigate the stability of different Brucella biotypes.
Main Methods:
- Examined 1,800 isolates using conventional identification techniques.
- Utilized manometric methods to assess oxidative metabolic patterns.
- Studied intra-strain variation by analyzing 50 colony-forming units from selected Brucella strains.
Main Results:
- Brucella melitensis and Brucella neotomae showed minimal intra-strain variation.
- Brucella abortus exhibited variants in dye sensitivity.
- Brucella suis displayed variations in conventional characteristics and metabolic patterns.
Conclusions:
- Brucella suis demonstrates considerable genetic and metabolic plasticity.
- Brucella abortus strains possess variability influencing their characteristics.
- Brucella melitensis and B. neotomae are genetically stable within the studied biotypes.