Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Control of pT181 replication II. Mutational analysis.

S Carleton, S J Projan, S K Highlander

    The EMBO Journal
    |October 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Managing the Diabetic Athlete.

    The Physician and sportsmedicine·2016
    Same author

    The complete genomes of Staphylococcus aureus bacteriophages 80 and 80α--implications for the specificity of SaPI mobilization.

    Virology·2010
    Same author

    Climate change and the integrity of science.

    Science (New York, N.Y.)·2010
    Same author

    MgrA is a multiple regulator of two new efflux pumps in Staphylococcus aureus.

    Journal of bacteriology·2005
    Same author

    Antibacterial drug discovery: is it all downhill from here?

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2004
    Same author

    Uses of Staphylococcus aureus GeneChips in genotyping and genetic composition analysis.

    Journal of clinical microbiology·2004
    Same journal

    Chromosome condensation mechanically primes the nucleus for mitosis.

    The EMBO journal·2026
    Same journal

    NDR kinase SAX-1 controls dendrite branch-specific elimination during neuronal remodeling in C. elegans.

    The EMBO journal·2026
    Same journal

    Assembly of the catalytic module and the rotor of human ATP synthase.

    The EMBO journal·2026
    Same journal

    Substrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidC.

    The EMBO journal·2026
    Same journal

    Conformational changes of the baseplate regulating tail contraction of Staphylococcus phage 812.

    The EMBO journal·2026
    Same journal

    Cellular assembly and functional resilience of the mammalian RNA exosome.

    The EMBO journal·2026
    See all related articles

    Researchers isolated mutations affecting Staphylococcus aureus plasmid pT181 replication. They identified recessive mutations impacting CopA RNA function and dominant mutations affecting the inhibitor

    Area of Science:

    • Molecular Biology
    • Microbial Genetics
    • Plasmid Biology

    Background:

    • Staphylococcus aureus plasmid pT181 replication is regulated indirectly through control of RepC protein synthesis.
    • CopA RNA acts as a primary negative control element, inhibiting RepC synthesis by binding to the repC mRNA leader.
    • This interaction likely forms a stem-loop structure, blocking ribosome binding and repC translation.

    Purpose of the Study:

    • To isolate and analyze mutations affecting the regulatory mechanisms of Staphylococcus aureus plasmid pT181 replication.
    • To investigate the roles of CopA RNA and its target in replication control.
    • To understand the genetic basis of both CopA RNA-sensitive and -insensitive mutations.

    Main Methods:

    • Isolation and characterization of recessive and dominant mutations affecting pT181 replication.

    Related Experiment Videos

  • Analysis of mutations within the copA gene and elsewhere on the plasmid.
  • Assessment of inhibitor-target interactions and plasmid compatibility.

    • Main Results:

    • Recessive mutants exhibited defects in CopA RNA structure or activity.
    • Dominant mutants showed alterations in the CopA RNA target site, with some mutations affecting both CopA RNA structure and its target.
    • Mutations outside the copA gene produced wild-type CopA RNA but altered target specificity.
    • pT181 copy mutants with wild-type inhibitor could be co-maintained with the wild-type plasmid.
    • Changes in inhibitor-target specificity did not alter plasmid incompatibility specificity.

    Conclusions:

    • The study elucidates the intricate regulatory network of pT181 plasmid replication involving CopA RNA and its target.
    • Identified mutations provide insights into the molecular mechanisms of RNA-mediated gene regulation and plasmid copy number control.
    • The findings contribute to understanding plasmid biology and potential strategies for manipulating plasmid maintenance.