[Redistribution of Ca2+ ions in lymphocyte suspensions after treatment with an ionophore]

Ionophore A23187 induces three-phase kinetics of reduction of chlorotetracycline (CTC) lauded lymphocyte fluorescence: fast reduction, low increase and lower reduction of fluorescence. We suggested the following explanation of kinetics. The first phase is extinguishing of the Ca2+ - CTC complex fluorescence induced by ionophore A23187 perturbation of the cell membrane, the second one is the energy dependent Ca2+ entry into the cell and the third one is the slow efflux of Ca2+ ions from the cell to concentration gradient.