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Age, strain and species differences in circulating parathyroid hormone.
Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|December 1, 1984
Summary
A new radioimmunoassay accurately measures intact parathyroid hormone (PTH) in rats. This sensitive assay reveals that circulating PTH levels increase with age in F344 rats.
Area of Science:
- Endocrinology
- Assay Development
- Animal Models
Background:
- Parathyroid hormone (PTH) is crucial for calcium homeostasis.
- Accurate measurement of intact PTH is essential for physiological studies.
- Existing assays may lack specificity or sensitivity for certain applications.
Purpose of the Study:
- To develop and validate a sensitive radioimmunoassay for intact rat parathyroid hormone (PTH).
- To characterize the assay's specificity and performance in rat serum.
- To investigate age-related changes in circulating PTH in F344 rats.
Main Methods:
- Development of a radioimmunoassay using chicken antibody C2-7, immunized with bovine PTH.
- Validation using rat serum and parathyroid gland extracts, assessing parallelism with bovine PTH.
- Testing assay specificity against synthetic PTH fragments and evaluating performance in response to EDTA infusion and thyroparathyroidectomy.
Main Results:
- The assay demonstrated high sensitivity (6 pg/tube) and specificity for intact PTH.
- Intra-assay (6.0%) and inter-assay (7.2%) coefficients of variation were low.
- Assay results correlated with physiological changes (EDTA, thyroparathyroidectomy) and revealed a novel age-dependent increase in PTH in F344 rats.
Conclusions:
- A sensitive and specific radioimmunoassay for intact rat PTH has been successfully developed and validated.
- The assay is suitable for measuring basal and stimulated PTH levels in rats.
- Circulating immunoreactive PTH progressively increases with aging in F344 rats, suggesting age-related endocrine changes.