[Interaction of methylmethacrylate and acrylamide with the microsomal oxidation system of the rat liver]

A complex, exhibiting the maximal absorption at 385-388 nm and minimal at 414-420 nm, was formed after interaction between cytochrome P-450 and methylmethacrylate and acrylamide at 5 mM concentration. Acrylamide at concentrations higher than 6 mM induced the formation of modified type 11 differential spectra with maxima at 418 nm and minima at 385 nm. Methylmethacrylate and acrylamide stimulated utilization of NADPH and inhibited hydroxylation of 3,4-benzpyrene. Methylmethacrylate inhibited also N-demethylation of aminopyrine. After treatment of rats with the monomers activities of cytochromes P-450, b5 and NADPH-cytochrome c reductase were unaltered. Methylmethacrylate and acrylamide appear to interact with the microsomal oxidative system.