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R68.45 plasmid mediated conjugation in Thiobacillus A2.
Microbios
|January 1, 1984
Summary
Broad host range IncP-1 plasmid R68.45 was successfully transferred to Thiobacillus A2. This plasmid enabled antibiotic resistance expression and facilitated the transfer of chromosomal markers in Thiobacillus A2.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- IncP-1 plasmids are known for their broad host range.
- Thiobacillus A2 is an important bacterium in environmental processes.
- Understanding plasmid transfer in Thiobacillus is crucial for genetic manipulation.
Purpose of the Study:
- To investigate the transfer and stability of the IncP-1 plasmid R68.45 into Thiobacillus A2.
- To assess the expression of antibiotic resistance genes from R68.45 in Thiobacillus A2.
- To evaluate the potential of R68.45 for mediating chromosomal gene transfer in Thiobacillus A2.
Main Methods:
- Conjugational transfer of plasmid R68.45 from Pseudomonas aeruginosa or Escherichia coli to Thiobacillus A2.
- Assessment of plasmid stability and antibiotic resistance gene expression in Thiobacillus A2.
- Observation of R68.45 transfer between Thiobacillus A2 strains and its ability to mediate chromosomal marker transfer.
Main Results:
- Plasmid R68.45 was stably maintained in Thiobacillus A2.
- Neomycin/kanamycin resistance was fully expressed in Thiobacillus A2.
- Conjugational transfer of R68.45 between Thiobacillus A2 strains occurred, and R plasmid mediated chromosomal marker transfer was demonstrated.
Conclusions:
- The IncP-1 plasmid R68.45 can be successfully transferred and stably maintained in Thiobacillus A2.
- R68.45 confers neomycin/kanamycin resistance in Thiobacillus A2 and can mediate chromosomal gene transfer, offering a tool for genetic studies.