[Binuclear neurons in the brain of hens in experimental pesticide poisoning]

The material for studies consisted of brains of broilers at the age of 7 weeks. The chickens were experimentally intoxicated with doses of some pesticides or their mixtures. Brains were taken from dead animals of the experimental groups and control chickens, which were fixed in neutralized formalin solution, and, after dehydrating and embedding them in paraffin, they were cut with the microtome into sections stained with hematoxylin and eosin and according to Klüver Barrer's method. In the material studied the presence of single binuclear neurons was found in all regions of the brains. The number of such cells considerably increased in some regions of the brain (Purkinje cell layer of the cerebellum, hypermetropic nucleus, thalamus) of experimentally intoxicated chickens and dead ones after 12 - 24 hr from poisoning. The results of the studies suggest the possibility of neuron division in the central nervous system of birds under the influence of the action of a pathogenic agent - pesticides as regards this paper.