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On inactivation of bacteriophage lambda by hydroxylamine.

Mutation Research

|February 1, 1980

Summary

Hydroxylamine mutagenicity is higher on single-stranded DNA. Surprisingly, the cohesive ends of lambda DNA, despite having few cytidine residues, are as reactive to hydroxylamine as the entire double-stranded DNA molecule.

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Hydroxylamine is a known mutagen.
Its mutagenic activity differs between single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA).

Purpose of the Study:

To investigate the reactivity of specific DNA structures to hydroxylamine.
To compare the hydroxylamine target size of ssDNA regions with dsDNA regions.

Main Methods:

Treatment of lambda DNA with hydroxylamine.
Analysis of hydroxylamine-induced mutations or modifications.

Main Results:

Hydroxylamine is significantly more active on ssDNA than dsDNA.
The cohesive ends of lambda DNA, containing 10 cytidine residues, represent a hydroxylamine target magnitude comparable to the entire duplex region (approx. 25,000 cytidine residues).

Conclusions:

The cohesive ends of lambda DNA are highly susceptible to hydroxylamine.
This suggests that specific structural features, not just sequence length, influence hydroxylamine mutagenicity.

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