[Sertoli cells of the impubescent pig: a method for obtaining isolated cells]

Minced whole testes from immature pigs were treated with collagenase (0.4 mg/ml) then with trypsin (2.5 mg/ml). The resulting suspension of cells was divided into sterile bottles containing medium 199 supplemented with 5 p. 100 calf serum. Bottles were maintained at 32 degrees C. After about 24 hours medium was changed 3 times to eliminate free cells. Within 3 days preparations consisting of 81.5% Sertoli cells, 0.5% Leydig cells and 18% peritubular cells were obtained.