Relationship between the house dust and mite allergen by immunochemical and clinical evidence

This investigation was carried out to determine antigenic potency in extracts of house dust, D. pteronyssinus and D. farinae and Aspergillus fumigatus with observation of precipiting antibodies in the serum of immunised rabbits. The titre of precipiting antibodies dependens on the mode of immunisation, preparation and concentration of antigens. Our investigations revealed that the house dust antigen comprises antigen of D. pteronyssinus, which can clarify the cross-reaction between the antiserum to the mite and antigens of the mite and house dust. The house dust allergen component is most probably present in second peak obtained by fractionation on Sephadex G 200, which was confirmed by double immunodiffusion with two precipitation lines. Fraction 2 of house dust is observed to bring about more pronounced skin reaction than fraction 1.