[Laparoscopic aspiration of Graafian follicle--its action on ovocyte ultrastructure (author's transl)]

The ultrastructure of human ovocytes was studied, the latter having been obtained laparoscopically following induced ovulation, using clomiphene and HCG. Optimum vacuum size for aspiration was tested on embryonated rat ova. It was found to be between 10 kPa and 30 kPa, that is between 100 and 300 mm H2O. Ultrastructural alteration was recordable neither from human nor from rat ovocytes. No change in ultrastructure was obtained either from subsequent in vitro culturing, and some of the ovocytes were embryonated with good success.