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Surface antigens specifically expressed by activated T cells in humans
European Journal of Immunology
|February 1, 1981
Summary
Researchers developed monoclonal antibodies (MLR 1-4) targeting activated T cells. These antibodies specifically identify T cells during immune responses, aiding in monitoring immune system diseases.
Area of Science:
- Immunology
- Cell Biology
Background:
- Activated T cells play a crucial role in immune responses.
- Monitoring T cell activation is vital for understanding immune system function and diseases.
Purpose of the Study:
- To generate and characterize novel monoclonal antibodies specific for activated human T cells.
- To investigate the expression patterns of antigens on activated T cells.
Main Methods:
- Immunization of BALB/c mice with activated human T cells from a secondary mixed lymphocyte reaction (MLR).
- Fusion of mouse spleen cells with P3X63Ag8U1 myeloma cells to produce hybridomas.
- Screening of hybridomas for monoclonal antibodies specific to activated T cells.
Main Results:
- Four monoclonal antibodies (MLR 1-4) were identified, specific for activated T cells and non-reactive with peripheral blood cells.
- The recognized antigens (MLR antigens) show differential distribution and temporal expression on stimulated T cells.
- MLR2 antibody stained nearly all responder T cells in secondary MLR, while MLR 1, 3, and 4 stained fractions.
- MLR2 and MLR3 were also found on mitogen-stimulated cells.
Conclusions:
- At least three MLR antigens can define distinct subsets of activated T cells.
- These monoclonal antibodies and their target antigens hold potential for monitoring T cell activation in various immune-related diseases.