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Related Experiment Videos

Rapid matching for transplantation: PHA primed lymphocytes.

F M Robbins, R J Hartzman

    Human Immunology
    |October 1, 1981
    PubMed
    Summary

    Human lymphocytes exhibit secondary in vitro restimulation (PLT) reactions that correlate with their cellular HLA-D type. This PLT assay may serve as a valuable cross-matching technique for kidney transplants.

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    Area of Science:

    • Immunology
    • Transplantation Immunology

    Background:

    • Human lymphocyte responses to phytohemagglutinin P (PHA-P) are crucial for understanding immune reactions.
    • Secondary in vitro restimulation (PLT) assays assess lymphocyte-mediated immune responses.
    • Human Leukocyte Antigen (HLA) typing is essential for transplantation compatibility.

    Purpose of the Study:

    • To investigate the correlation between human lymphocyte PLT reaction patterns and cellular HLA-D type.
    • To evaluate the utility of the PLT assay as a cross-matching technique for cadaveric renal transplantation.

    Main Methods:

    • Human lymphocytes were treated with PHA-P and assessed for PLT reactions after 10 days.
    • Cellular (HLA-D) typing was performed on the lymphocytes.
    • A standard 3H thymidine incorporation technique was used to measure lymphocyte stimulation, with incubation times as short as 12 hours.

    Main Results:

    • PLT reaction patterns directly corresponded to the lymphocytes' cellular HLA-D type.
    • Lymphocytes with shared HLA-D types elicited a weaker stimulation response compared to those with unshared types.
    • The PLT assay detected responses within 12 hours, though 22-48 hours was typical.

    Conclusions:

    • The PLT assay effectively reflects cellular HLA-D type in human lymphocytes.
    • The PLT assay demonstrates a strong correlation with primary mixed leukocyte responses.
    • The PLT assay shows promise as a simplified and effective cross-matching method for cadaveric renal transplantation.

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