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Mitomycin C- and streptozotocin-induced motility and numerical sperm variants in mice
Abstract:
Groups of HA (ICR) albino male mice were injected once i.p. with o, 2.5 or 5.0 mg/kg mitomycin C (MC), or 2.65 or 26.5 mg/kg streptozotocin (SZ). 4 weeks after treatment each male was mated with 2 untreated CF1 albino females. 5 weeks after treatment the males were killed for determination of sperm motility and count from the cauda and vas. Both agents decreased sperm motility and count in a dose-dependent manner in the treated males. The effect is believed to be caused primarily by interference with gene expression involved in spermatogenesis. The females mated to treated males showed decreased percentage pregnancy and had few progeny. Mean percentage motility and sperm count was significantly lower in the F1 male progeny of MC- or SZ-treated males compared to control males. 50-60% of F1 males form treated male parents had percentage sperm motility below control range. Decreased motility and count in the F1 progeny of treated males may have been caused by gene mutations and/or chromosomal aberrations. The method which is presented may be useful for detecting mutations and/or developmental effects.
Insights
Exposure to mitomycin C (MC) and streptozotocin (SZ) reduced sperm quality in male mice. Offspring of treated males also showed decreased sperm motility and count, suggesting heritable genetic damage.
Area of Science:
- Toxicology
- Reproductive Biology
- Genetics
Background:
- Spermatogenesis is a complex process susceptible to environmental toxicants.
- Assessing the impact of chemical exposure on male reproductive health is crucial.
Purpose of the Study:
- To investigate the effects of mitomycin C (MC) and streptozotocin (SZ) on mouse sperm parameters.
- To evaluate the potential for transgenerational effects on sperm quality in offspring.
Main Methods:
- Male mice were administered varying doses of MC or SZ.
- Sperm motility and count were assessed in treated males and their F1 progeny.
- Pregnancy rates and progeny counts were recorded.
Main Results:
- Both MC and SZ significantly reduced sperm motility and count in a dose-dependent manner.
- Females mated to treated males exhibited lower pregnancy rates and fewer offspring.
- F1 male offspring from treated fathers showed significantly reduced sperm motility and count, with many falling below control ranges.
Conclusions:
- MC and SZ interfere with spermatogenesis, likely by affecting gene expression.
- Heritable effects, including gene mutations and chromosomal aberrations, may occur in offspring.
- This study presents a potential method for detecting chemical-induced mutations and developmental effects.