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[Reactive SH-groups in clinical practice. Methodological notes].

G F Ferraccioli, A Baldini, U Ambanelli

    Acta Bio-Medica De L'Ateneo Parmense : Organo Della Societa Di Medicina E Scienze Naturali Di Parma
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

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    Accurate serum thiol (-SH) group determination requires controlling for anticoagulants like heparin and citrate, and light exposure. Standardized methods ensure reliable measurement of thiol reserves for assessing oxidative stress in disease.

    Area of Science:

    • Biochemistry
    • Clinical Chemistry

    Context:

    • Serum thiol (-SH) group levels are important biomarkers.
    • Extrinsic factors can significantly affect their measurement.
    • Accurate determination is crucial for understanding oxidative stress.

    Purpose:

    • To investigate extrinsic factors influencing serum -SH group determination.
    • To identify optimal conditions for reliable thiol level measurement.
    • To assess the impact of anticoagulants, light, and specific drugs on -SH levels.

    Summary:

    • Anticoagulants (heparin, citrate) and light exposure alter serum -SH levels; serum samples provide more reproducible data than plasma.
    • Non-steroidal anti-inflammatory drugs had no effect, while D-Penicillamine and Tiopronin transiently increased -SH levels (15-20%).

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  • Established methodological criteria enable reliable assessment of serum thiol reserve, relevant to free radical consumption in disease.

    • Impact:

    • Provides a standardized methodology for accurate serum -SH group determination.
    • Highlights the importance of sample handling (anticoagulant, light) for reliable results.
    • Supports the clinical utility of serum thiol measurements in evaluating oxidative-peroxidative processes and disease states.