Immune responses in vitro. XII. Two independently segregating loci control Mis product(s)

Distribution analyses of Misa in (BALB/c x DBA/2)F2 animals indicate that two independently segregating loci control the expression of Misa products. The results were dependent upon the use of x-irradiated cells a the antigen in MLR. The use of non-x-irradiated antigen in the MLR led to results that were uninterpretable because both DBA/2 and (BALB/c x DBA/2)F1 cells responded to x-irradiated BALB/c cells. Even though DBA/2 cells did not respond to (BALB/c x DBA/2)F1 cells, their reactivity to BALB/c cells indicates that the Misb of BALB/c is not null.