Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Estrogen receptor transformation to a high-affinity state without subunit-subunit interactions.

D Sakai, J Gorski

    Biochemistry
    |July 17, 1984
    PubMed
    Summary

    Monomeric rat estrogen receptors can transform into a high-affinity state upon heating, exhibiting altered chromatographic properties and slow estradiol dissociation, suggesting a conformational equilibrium. This transformation is independent of other cytosolic components.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Precise Spectroscopy of the 3n and 3p Systems via the ^{3}H(t, ^{3}He)3n and ^{3}He(^{3}He, t)3p Reactions at Intermediate Energies.

    Physical review letters·2024
    Same author

    Functional cell phenotype induction with TGF-β1 and collagen-polyurethane scaffold for annulus fibrosus rupture repair.

    European cells & materials·2020
    Same author

    Variability in the time to initiation of CPR in continuously monitored pediatric ICUs.

    Resuscitation·2018
    Same author

    Transplantation of activated nucleus pulposus cells after cryopreservation: efficacy study in a canine disc degeneration model.

    European cells & materials·2016
    Same author

    Intervertebral disc repair with activated nucleus pulposus cell transplantation: a three-year, prospective clinical study of its safety.

    European cells & materials·2015
    Same author

    Fibrin-genipin adhesive hydrogel for annulus fibrosus repair: performance evaluation with large animal organ culture, in situ biomechanics, and in vivo degradation tests.

    European cells & materials·2014

    Area of Science:

    • Biochemistry
    • Molecular Endocrinology
    • Protein Conformation Dynamics

    Background:

    • Estrogen receptor (ER) exists as aggregates, and its transformation is crucial for its function.
    • Understanding the monomeric state's behavior is key to elucidating ER activation mechanisms.

    Purpose of the Study:

    • To investigate the heat-induced transformation of monomeric rat estrogen receptor.
    • To determine if monomeric receptors can adopt properties of the transformed state independently of other factors.

    Main Methods:

    • Generating monomeric rat estrogen receptor by dissociating aggregates with KCl and hydroxylapatite binding.
    • Incubating purified monomeric receptor at 28-30°C after washing to remove cytosolic components.
    • Analyzing receptor properties using hydroxylapatite chromatography and estradiol dissociation kinetics.

    Related Experiment Videos

    Main Results:

    • Monomeric estradiol-receptor complexes transform into a state with coeluting chromatographic properties and slow estradiol dissociation.
    • Monomeric receptors on hydroxylapatite do not exhibit cooperative ligand binding, indicating a lack of dimerization or interaction with other proteins.
    • Sedimentation analysis confirmed the eluted receptor remained in the monomeric 4S form.

    Conclusions:

    • Monomeric estrogen receptors can transition to a high-affinity, slow-dissociating state independently.
    • Receptor monomers exist in an equilibrium between low- and high-affinity conformations.
    • Dimerization of high-affinity monomers may stabilize the transformed state.