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[Spoilage microorganisms encountered in ultra-high temperature processed milk]
Summary
Spoiled milk contained various microorganisms, primarily Bacillus species, suggesting contamination during the filling process. These microbes can thrive in different temperature conditions, impacting milk quality.
Area of Science:
- Food Microbiology
- Dairy Science
- Microbial Spoilage
Background:
- Ultra-high temperature (UHT) processed milk is expected to have a long shelf life.
- Microbial spoilage can occur despite processing, leading to economic losses.
- Identifying spoilage microorganisms is crucial for preventing future contamination.
Purpose of the Study:
- To identify the microorganisms responsible for the spoilage of UHT processed milk.
- To determine the potential sources of contamination in milk production.
- To characterize the temperature tolerance of isolated Bacillus strains.
Main Methods:
- Isolation and identification of aerobic and facultative anaerobic microorganisms from spoiled UHT milk.
- Microbial identification using standard microbiological techniques.
- Analysis of isolated strains for temperature growth characteristics (mesophilic, thermophilic, psychrotrophic).
Main Results:
- 40 microbial strains were isolated from spoiled UHT milk, including 13 Bacillus species (B. cereus, B. licheniformis, B. brevis, B. pumilus) and 27 non-sporeforming bacteria and yeasts.
- The identified microorganisms suggest contamination likely occurred during the filling operation.
- Isolated Bacillus strains exhibited mesophilic, thermophilic, and psychrotrophic properties.
Conclusions:
- Contamination during the filling stage is the primary cause of spoilage in UHT processed milk.
- The diverse temperature adaptability of Bacillus strains contributes to milk spoilage.
- Effective control measures during filling are essential to maintain UHT milk quality.