[Biphase reaction of enzymatic pyruvate reduction during inhibition]

At least two stages have been revealed in the reaction of enzymatic reduction of pyruvate under inhibition. A fast process during the first stage of the reaction was finished in a period of "dead time" with routine spectroscopic measurements. A reaction rate at this stage decreased by three times. The second stage was characterized by a constant rate, which changed by less than 10%. The analysis of neutral salt influences on the inhibition complex showed, that the latter decomposed rapidly. A model suggested earlier, was used for interpreting the reversible product inhibition of the reaction catalysed by LDH.