Effect of albumin immobilization by plasma polymerization on platelet reactivity

Albumin was immobilized on polyethylene (PE) film by the plasma polymerization method. Immobilized albumin on formaldehyde polyermized PE film was hard to desorb even when the film was dipped in blood plasma. Amounts of platelet adhesion on the film and serotonin release decreased clearly. Aggregated platelets were observed on PE film but not observed on the albumin immobilized surface. These results seemed to be due to not only immobilized albumin, but also the surface charge determined by plasma protein adsorption. That is, the slightly negatively charged surface indicated good anti-thrombogenicity.