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Atropine esterase status of laboratory mice
Laboratory Animal Science
|August 1, 1984
Summary
Laboratory mice lack significant atropine esterase levels, unlike rabbits. This indicates atropine esterase is not a polymorphic variant in mice under tested conditions.
Area of Science:
- Biochemistry
- Genetics
- Pharmacology
Background:
- Atropine esterase is an enzyme found in some species, notably rabbits, where it exhibits polymorphic variation.
- Understanding the presence and variation of atropine esterase in different species is crucial for pharmacological and toxicological studies.
Purpose of the Study:
- To investigate the presence and levels of atropine esterase in various laboratory and wild mouse strains.
- To determine if atropine esterase exhibits polymorphic variation in mice, similar to observations in rabbits.
Main Methods:
- Tested 58 inbred laboratory mouse strains and two wild mouse species (Mus castaneus and Peromyscus maniculatus bairdii).
- Utilized the microhematocrit tube test and the agar plate test to detect atropine esterase activity.
Main Results:
- Laboratory mice strains did not show significant levels of atropine esterase activity by either testing method.
- Wild mouse species were also assessed, though the primary focus was on laboratory strains' lack of significant enzyme levels.
Conclusions:
- Atropine esterase is not present at significant levels in the tested laboratory mouse strains.
- The findings suggest that atropine esterase is not a polymorphic variant in laboratory mice under the evaluated conditions, contrasting with findings in rabbits.