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Updated: Aug 13, 2026

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
Published on: August 19, 2013
Fluorometric determination of malonic acid with dicyclohexylcarbodiimide
Abstract:
By using dicyclohexylcarbodiimide (DCC) as a reagent, a fluorometric method for the determination of malonic acid has been developed. The reagent combined with the acid to afford N,N-dicyclohexylbarbituric acid which reacted with glutaconic aldehyde, generated from the reaction of DCC with pyridine, to form a fluorophore. The fluorophore was then determined at lambda ex = 469 nm and lambda em = 562 nm. The calibration curve was linear from 2.5 to 25 nmol. The relative standard deviation obtained with 10 nmol of malonic acid was 5.77% (n = 10). The specificity is also discussed.
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