[Introduction of the inoculum into the upper agar layer as an alternative method in determining microorganism...

A procedure for addition of the inoculum to the surface layer of semisynthetic agar was developed and recommended for the use in the assay of antibiotic sensitivity of microorganisms. The procedure differs from the alternative method of Barry et al. It is of special value when it is necessary to determine antibiotic sensitivity of the pathogens of highly contagious infections with the agar diffusion method.