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Updated: Jul 23, 2026

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
Published on: February 21, 2011
A simple machine for smoke analytical studies and total particulate matter collection for biological studies
Abstract:
The need for modest quantities of smoke for use in biological studies resulted in the development of a simple machine for smoke analytical studies and total particulate matter (TPM) collection. The machine can be used for the study of mainstream and sidestream smoke and the gas phase of both, with the accuracy and precision of more complex smoking machines. The machine is described and machine performance is evaluated by TPM data obtained under typical operating conditions. One gram of TPM can be collected from a high TPM delivery cigarette by one technician in 2 h. When sidestream smoke is collected, the machine can be operated without the escape of smoke components to the laboratory atmosphere.
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