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Cells that mediate NK like cytotoxicity are present in the human delayed type hypersensitivity response
Clinical and Experimental Immunology
|November 1, 1984
Summary
Natural killer (NK) like cells are present in human delayed type hypersensitivity (DTH) skin responses. These stimulated mononuclear cells exhibit cytotoxicity against both NK-sensitive and NK-resistant target cells.
Area of Science:
- Immunology
- Cellular Biology
Background:
- Delayed type hypersensitivity (DTH) is a critical immune response.
- Natural killer (NK) cells are key players in innate immunity and cytotoxicity.
Purpose of the Study:
- To investigate the presence and function of NK-like cytotoxic cells within human DTH responses.
- To characterize the phenotype of these DTH-associated killer cells.
Main Methods:
- Induction of DTH responses in human skin blisters.
- Isolation of cells from DTH-associated and non-DTH skin blisters.
- Assessment of cytotoxic activity against K-562 (NK-sensitive) and Daudi (NK-resistant) target cells.
Main Results:
- Cells mediating NK-like cytotoxicity were identified within DTH responses.
- Significantly higher cytotoxic activity was observed in cells from DTH blisters compared to non-DTH blisters.
- The identified killer cells were mononuclear and demonstrated enhanced cytotoxicity against both NK-sensitive and NK-resistant cell lines, suggesting activation.
Conclusions:
- Human DTH responses involve the recruitment and activation of cells with NK-like cytotoxic capabilities.
- These DTH-associated cytotoxic cells are likely stimulated mononuclear cells with a broad target range.