[Repaired and unrepaired DNA damage induced by x-irradiation of nonproliferating rat hepatocytes]

An effective system of repair of double-strand DNA breaks was found in the stock culture of rat hepatocytes by the method of elastoviscosimetry. The inhibitors of DNA resynthesis, hydroxyurea and arabinosyl cytosine, which transformed single-strand breaks into double-strand ones were used to estimate the level of spontaneous affection of the hepatocyte genome. With a reference to this level the authors have demonstrated that after a dose of 2 Gy and 6-24-hour repair, the additional number of lesions was registered. These lesions were shown to be incised by nucleases similar to those involved into the conventional repair process.