The healing of rabbit corneal endothelium

A circular 4 mm endothelial defect was induced by transcorneal freezing. The experimental damage and the healing took place in the living rabbit in 15 eyes, and in the isolated cornea in organ culture in further 20 eyes. The reparative process was studied by SEM, and proved to be the same in vivo and in vitro. The defect was covered with endothelial cells after 3 days. The normal hexagonal pattern was regained after 3 weeks. Both cell migration and cell division were involved in the reparative process. Only cells recruited from a zone close to the defect were active; the cells situated more than a few cell diameters from the original edge maintained their form and size unchanged. The first phase of cell division was the formation of a spherical cell with numerous blebs on its surface.