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Detection of aflatoxin D1 in ammoniated corn by mass spectrometry-mass spectrometry
Applied and Environmental Microbiology
|October 1, 1984
Abstract:
Corn cultured with Aspergillus flavus to produce a high level of aflatoxin was ammoniated at 37 degrees C for 21 days. An extract of the ammoniated corn was separated by thin-layer chromatography, followed by reversed-phase high-pressure liquid chromatography. Examination of the fractions by tandem mass spectrometry led to detection of aflatoxin D1 as a product of the corn ammoniation process.